Waters Ostro Sample Preparation Products Instrukcja Użytkownika Pobierz pdf

[ Care and Use ManUal ]

1Ostro Sample Preparation Plate

Contents

I. INTRODUCTION

II. PROCEDURE

III. ORDERING INFORMATION

I. INTRODUCTION

Ostro

™

sample preparation plates provide a novel approach for the

removal of phospholipids from biological samples. The Ostro 96-well

plate design uses an in-well protein precipitation in combination

with a single, rapid, pass-through method that provides consistent,

high extraction recoveries for acids, bases, and neutrals. Designed

specifically to meet the needs of the bioanalytical scientist, Ostro

enhances a laboratory’s performance by providing greater repro-

ducibility, improved recovery, and increased phospholipid

removal when compared with competitive techniques.

A straightforward 6-step procedure was designed to facilitate in-well

protein precipitation and produce filtrates free of proteins and other

endogenous components. These filtrates are then suitable for high-

throughput LC/MS/MS analysis.

II. PROCEDURE

Step 1: Preparation

Place the Ostro plate onto a 2 mL collection plate for processing.

Step 2: Plasma Loading

Add between 50 and 200 μL of plasma sample into the wells. The

Ostro plate is designed to prevent plasma samples from leaking

through the plate.

Step 3: Precipitation

Solvent Loading

Forcefully/rapidly add 1% HCOOH (formic acid) in acetonitrile to the

wells in a ratio of 3:1 solvent to plasma.

Note: The forceful/rapid addition of the solvent aids in mixing the

plasma with this solvent.

Note: Take care to ensure that all of the pipette tips are properly

positioned in the samples well to prevent any loss of mixing of samples.

ostro sample preparation plate

96-Well 2 mL Collection Plate

Ostro Plate

1 2 3

Podsumowanie treści

Strona 1 - [ Care and Use ManUal ]

[ Care and Use ManUal ]1Ostro Sample Preparation PlateContentsI. INTRODUCTIONII. PROCEDUREIII. ORDERING INFORMATIONI. INTRODUCTIONOstro™ sample

Strona 2

[ Care and Use ManUal ]2Ostro Enhanced Protein Precipitation PlateStep 4: MixingAspiration (Preferred)Aspirate the samples up and down 3X by a manual

Strona 3 - Sales Offices

[ Care and Use ManUal ]3Ostro Sample Preparation PlateIII. ORDERING INFORMATIONDescription Volume Qty/Pkg Part NumberOstro 96-Well Plate, 25 mg 1 1860

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